Organización Internacional del Bambú y el Ratán

Organización Internacional del Bambú y el Ratán

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In vitro regeneration of bamboos, the «Green Gold»: An overview

Artículos

Revista/Conferencia:

INDIAN JOURNAL OF BIOTECHNOLOGY

Language:

English

Autor:

Goyal Arvind Kumar

Experts:

Sen Arnab

Año:

2016

Volumen:

15

Edición:

1

Número de páginas:

9-16

Palabras claves:

Bamboo; micropropagation; plant growth regulators (PGRs); regeneration; rhizogenesis

Bamboo is a versatile, non-timber, forest product having glorious history. Being a vital resource, there is an ever increasing demand for this ‘Green Gold’, but conventional breeding is severely handicapped because of two main reasons, i.e., long vegetative phase and irregular flowering. Thus, to fulfill the requirement of demand, the best way is to switch to scientific techniques. In vitro culture offers an alternative option for producing desirable clones in stipulated period of time. Among the different explants used for micropropagation of bamboos, young branch node was the most preferred. Murashige and Skoog’s medium supplemented with sucrose and plant growth regulators (PGRs) were extensively used in the regeneration of bamboos. PGRs like 6-benzylaminopurine (BAP), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), kinetin (Kn), 1-naphthaleneacetic acid (NAA), 2,4 dichlorophenoxyacetic acid (2,4-D) were mostly preferred over others for bamboo micropropagation, while 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) and gibberellic acid (GA(3)) were also used in some cases. Direct in vitro shooting was preferred in regeneration of bamboos over somatic embryogenesis through callus culture. In vitro rhizogenesis was well achieved in presence of phytohormones but was not solely depended on them. Further, regenerated plantlets were successfully established in the field with high rate of survival. In the present review we endeavor to give a consolidated account of in vitro regeneration of bamboos starting from the pioneer work initiated in 1968.