The comfort characteristics of bamboo/cotton-blended knitted fabrics made from rotor-spun yarns were studied in this research work. Five different blend proportions namely 100% bamboo, 100% cotton, 50:50 cotton:bamboo, 70:30 cotton:bamboo and 30:70 cotton:bamboo were used to produce rotor-spun yarns of 30(s)Ne(c). The yarn samples developed out of these different blend proportions were evaluated for yarn strength and elongation, yarn unevenness, yarn imperfections and used to produce single-jersey-knitted fabrics. All the fabric samples were dyed and finished. Fabric samples were evaluated for geometrical properties such as course/inch, wales/inch, stitch density and thickness apart from measuring comfort properties such as airpermeability, thermal conductivity, thermal resistance and water vapour permeability. The results indicated that 50/50 bamboo/cotton-blended knits have comparable fabric quality in terms of comfort properties with respect to 100% bamboo fabrics.