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Acute and 28-day Sub-acute Oral Toxicity Evaluation of Two Dietary Bamboo Charcoal Powders in Sprague-Dawley Rats

Articles

Journal/Conference:

JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES

Language:

English

Author:

Jia Zhenchao; Luo Sha; Zhong Yuting; Li Xiao; Chen Jinyao

Experts:

Zhang Lishi

Year:

2015

Volume:

35

Issue:

2

Pages:

192-199

Keywords:

bamboo charcoal powder; size distribution; acute toxicity; 28-day sub-acute toxicity

No data were available on the acute oral toxicity, short-term oral toxicity of vegetable carbon in animals. This study was designed to evaluate the safety of two commercially available dietary bamboo charcoal powders (BCP1 and BCP2). The size distribution of the two powders was determined by a Mastersizer 2000 laser particle size analyzer prior to the in vivo safety studies. For the acute toxicity study, a single dose of 11.24 g/kg body weight of BCP1 and BCP2 was given once orally to healthy Sprague-Dawley (SD) rats. Mortality and clinical symptoms were observed and recorded for the first 30 min after treatment, at 4 h post-administration, and then at least once daily for 14 days after administration. In the repeated dose 28-day oral toxicity study, BCP1 and BCP2 were administered orally at doses of 2.81, 5.62, and 11.24 g/kg body weight for 28 days to SD rats. Animals were sacrificed and organs and blood samples were analyzed. Results showed that both BCP1 and BCP2 were micro-sized and various in size. In the acute toxicity and the repeated dose 28-day oral toxicity studies, BCP caused neither mortality nor visible signs of toxicity in rats. No significant differences were found in the relative organ weights or in biochemical parameters in BCP treated groups compared to a control group. No treatment-related histological changes were observed in the organs of these animals. Based on these data, it is concluded that the median lethal dose (LD50) of BCP for both male and female rats is more than 11. 24 g/kg body weight and the no-observed-adverse-effect level (NOAEL) is > 11.24 g/kg body weight for 28 days.