International Bamboo and Rattan Organization

International Bamboo and Rattan Organization

Advanced search

-
Back

Bamboo Leaf Flavonoids Alleviates Isoflurane-induced Cognitive Impairment in Aged Rats

Articles

Journal/Conference:

PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS

Language:

English

Author:

Liu YanFeng

Experts:

Liu Zhan

Year:

2016

Volume:

43

Issue:

7

Pages:

699-705

Keywords:

bamboo leaf flavonoids; isoflurane; cell apoptosis; cognitive impairment

To examine the effect of bamboo leaf flavonoids on isoflurane-induced neuronal apoptosis and cognitive impairment in aged rats. The primary hippocampal neurons were isolated and cultured. After hippocampal neurons were treated with 2% isoflurane for 6 h, and treated with 50, 80 and 100 mg/L of bamboo leaf flavonoids, the cell proliferation was measured by MTT method and cell apoptosis was measured by flow cytometry. The rats were inhaled with 1.4% isoflurane for 2 h, and injected intraperitoneally with 25, 50 and 100 mg/kg of bamboo leaf flavonoids for 5 consecutive days. The cognitive functions were examined by Morris water maze. The hippocampus were isolated for the determinations of A beta(1-42), TNF-alpha and INF-gamma by ELISA. Results showed that bamboo leaf flavonoids significantly increased the proliferation of hippocampal neurons and suppressed hippocampal neurons apoptosis compared with isoflurane treatment only. The result of Morris water maze showed that bamboo leaf flavonoids significantly decreased average escape latent period and increased the frequency of crossing platform. The results of ELISA showed that bamboo leaf flavonoids suppressed the expression of A beta(1-42), TNF-ot and INF-gamma in hippocampus. Our findings suggested that bamboo leaf flavonoids enhanced immunity of aged rats and suppressed the expression of A beta(1-42), leading to the inhibition of isoflurane-induced neuronal apoptosis and cognitive impairment.