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BIOCONVERSION OF BAMBOO GRASS CULM HYDROLYZATE INTO XYLITOL BY YEAST CANDIDA MAGNOLIAE

Articles

Journal/Conference:

CELLULOSE CHEMISTRY AND TECHNOLOGY

Language:

English

Year:

2015

Volume:

49

Issue:

9-10

Pages:

807-812

Keywords:

Sasa senanensis; bamboo grass; hemicellulose hydrolyzate; xylitol; Candida magnoliae

A sugar solution containing 32 g dm(-3) of xylose was prepared from the culm of Sasa senanensis by hydrolysis with 2% sulfuric acid with a liquid-to-solid ratio of 5 (g g(-1)) at 120 degrees C for 1 h. During the acid hydrolysis, some byproducts were generated, such as acetic acid, furfural and low molecular weight phenols, which inhibit bioconversion of xylose to xylitol. Except for acetic acid, these inhibitors were successfully removed from the hydrolyzate by contacting with a steam-activated charcoal (25 g dm(-3) dose) for 1 h. Bioconversion of the detoxified hydrolyzate to xylitol by the yeast Candida magnoliae was investigated under the microaerobic conditions. The oxygen transfer rate (OTR) varied from 12.6 to 15.1 mmol-O-2 dm(-3) h(-1). The best fermentative performance of C. magnoliae in the culm hydrolyzate of S. senanensis (xylitol yield of 0.55-0.60 g-xylitol g-xylose(-1) and volumetric productivity of 0.82 g dm(-3)h(-1)) was obtained at the OTR ranging 14.1-15.1 mmol-O-2 dm(-3) h(-1).