Bambusa nagalandiana Naithani is an indigenous bamboo found in Nagaland and Mizoram, India. The species is a sympodial bamboo and identified by its yellow culm with green stripes. Because of its beautiful architecture the species is used for ornamental purpose, construction work and furniture making. The young shoots are also used as vegetable. Due to narrow distributional range and high demand for various purposes the species is under threat in the natural habitat. Present communication reports a successful attempt to develop protocol for macropropagation from culm segments and branch cuttings of this economically important threatened bamboo species.