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ISOLATION OF CULTURABLE ENDOPHYTIC BACTERIA FROM MOSO BAMBOO (PHYLLOSTACHYS EDULIS) AND 16S RDNA DIVERSITY ANALYSIS

Articles

Journal/Conference:

ARCHIVES OF BIOLOGICAL SCIENCES

Language:

English

Author:

Yuan ZongSheng; Liu Fang

Experts:

Zhang GuoFang

Year:

2015

Volume:

67

Issue:

3

Pages:

1001-1008

Keywords:

Phyllostachys edulis; culturable endophytic bacteria; 16S rDNA; diversity

We analyzed culturable endophytic bacteria from Moso bamboo (Phyllostachys edulis) using traditional bacterial isolation and culture methods and then studied the colony characteristics and diversity with a 16S rDNA sequence analysis. We isolated 82 endophytic bacteria strains belonging to 47 species in 26 genera from the root, rhizome, stem and leaves of Moso bamboo species from populations on Wuyi Mountain, and in the Jiangle and Changting regions. There were significant differences in the composition of the culturable endophytic bacteria isolated from the different areas and from different tissues. The dominant bacteria strains from the Wuyi Mountain samples were Arthrobacter, Staphylococcus, Bacillus and Enterobacter, while the dominant bacteria from the Jiangle samples were Bacillus, Staphylococcus and Curtobacterium, and the dominant bacteria in the Changting samples were Alcaligenes, Pseudomonas, Staphylococcus and Bacillus. Our results demonstrate the abundant diversity of endophytic bacteria in Moso bamboo.